Regulatory
Part:BBa_K1204024:Design
Designed by: Matthew Baron Group: iGEM13_Duke (2013-09-16)
TEF1pr-BS3x-20C
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 573
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 573
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 573
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 573
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 174
Design Notes
Had to consider binding rules of iTALs and gRNA/dCas9 complex. Also had to create spacers between multiple binding sites.
Source
Ordered as gene block from IDT